Postbac
NIST has developed a new method to create and validate an absolute cell counting reference. The project plan is to complete validation and robustness tests of the method across various counting procedures, including generalization of a mathematical model that extracts uncertainties associated with each step of the measurement process and the counting instrument itself. Briefly, the successful candidate will develop robust protocols, validate configurations (e.g., concentration(s), media composition, and particle type), and establish guidance that enables adaptation to other particle types and cells. This project supports a recently funded NIST Emerging Technology Standards Engagement (NESTE) project, which promotes U.S. measurement science in support of the bioeconomy.
A Platform for Uncertainty Quantification of Absolute Particle Count
- A B.S. Degree in Biomedical Engineering, biology, or equivalent demonstration of experience and/or coursework
- Some experience in a research laboratory preferred
- Experience with optical microscopes and image analysis
- Experience with microfluidic device fabrication
- Familiarity with flow cytometry and sorting
- Ability to develop computational tools for data analysis.
- Strong oral and written communication skills.
Key responsibilities will include but are not limited to:
- Maintenance of mammalian cell cultures, fluorescence staining, immunohistochemistry
- Fluorescence microscopy and image analysis
- Operation of analytical and sorting flow cytometers and data analysis from samples
- Utilizing methods such as photolithography, soft-lithography, alignment and bonding, curing, and surface modifications to make microfluidic devices with integrated waveguides
- Presenting results at internal meetings, and occasional meetings with external stakeholders
- Ensuring that results, protocols, software, and documentation have been properly archived